Protein Purification Reagents and Kits

Protein Purification Reagents and Kits
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Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells
Isolate intact mitochondria from cultured mammalian cell samples in about 40 minutes with this versatile, benchtop microcentrifuge-based fractionation kit.

Thermo Scientific™ HisPur™ Ni-NTA Resin
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Stationary Phase | Divalent Nickel, Metal Chelate |
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Type | Resin |
Target | His-Tagged Protein |
Content And Storage | Store at 4°C |
Format | Bottle |
Description | HisPur Ni-NTA Resin |
Form | Liquid Suspension |
Column Type | Affinity, Agarose Resin |
Capacity (Metric) | Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin |
Product Line | HisPur™ |
Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells
Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose
Thermo Scientific™ Pierce™ Anti-DYKDDDK Magnetic Agarose Beads provide a fast, convenient method for purification of DYKDDDK-tagged recombinant proteins

dimMatrix | Magnetic Agarose |
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Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents
Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

Buffer | Lysis Buffer |
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Shipping Condition | Ambient |
Form | Liquid |
Product Type | Nuclear and Cytoplasmic Extraction Reagent |
Reagent Type | Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation |
Product Line | NE-PER™ |
Thermo Scientific™ Pierce™ 3x DYKDDDDK Peptide
Tricosa-peptide for competitively eluting DYKDDDK-tagged fusion proteins from immobilized supports

Shipping Condition | Wet Ice |
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Format | Lyophilized Powder |
Form | Lyophilized Powder |
Protein Form | Recombinant |
Product Type | Peptides |
dimMatrix | Affinity Resin |
For Use With (Application) | Protein Purification and Isolation |
Product Line | Pierce™ |
Thermo Scientific™ CaptureSelect™ Human IgG-Fc PK Biotin Conjugate
Thermo Scientific™ CaptureSelect™ Human IgG-Fc PK Biotin Conjugate consists of a 13 kDa recombinant single domain antibody fragment that specifically binds to the Fc part of all four human IgG subclasses without cross-binding to mouse, rat, rhesus, and cynomolgus monkey IgG.

Ligand Type | Antibody |
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Type | Conjugate |
Content And Storage | Store in freezer (-5°C to -30°C). |
Form | Liquid |
Label or Dye | Biotin & Other Haptens |
Product Line | CaptureSelect™ |
Thermo Scientific™ CaptureSelect™ CH1-XL Ligand Leakage ELISA
Thermo Scientific™ CaptureSelect™ CH1-XL Ligand Leakage ELISA Kit is designed to detect possible leached ligand of antibodies or antibody fragments purified using CaptureSelect CH1-XL Affinity Matrix.

Shipping Condition | Dry Ice |
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Type | ELISA Kit |
Content And Storage | Store in freezer (-5°C to -30°C). |
Format | Kit |
Product Line | CaptureSelect™ |
Cytiva Superose™ 6 Increase Columns
High-resolution size-exclusion chromatography for studying large proteins and complexes. Cytiva Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).

Particle Size | 8.6μm |
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Ligand Type | Cross-linked agarose |
Content And Storage | 4° to 30° in 20% ethanol |
pH Range | 3 to 12 |
Column Type | Gel Filtration Column |
Material | Borosilicate glass |
Max. Pressure | 435 psi |
Cytiva PD MiniTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

Cytiva His GraviTrap™ Columns
Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.

Cytiva Ni Sepharose™ High Performance Media
Precharged with Ni2+ for optimal purification of histidine-tagged proteins. Cytiva Lifescience™ Ni Sepharose™ High Performance Media shows high binding capacity and compatibility.

Particle Size | 34μm |
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Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | Ni Sepharose HP chromato graphy media |
dimMatrix | Ni-Sepharose |
Support Type | 6% Cross-linked Agarose |
Cytiva HisTrap™ FF Crude Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Particle Size | 45 to 165 μm |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Flow Rate | <20 mL/min. |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Diameter (English) Inner | 0.6 in. |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |