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Invitrogen™ Dynabeads™ mRNA DIRECT™ Purification Kit

The Dynabeads™ mRNA DIRECT™ Kit is designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues.

Marca:  Invitrogen™ 61012

1105.80 EUR valid until 2025-04-30
Use promo code "24155" to get your promotional price.


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Codice prodotto. 10563755

  • 1224.00 € / Each

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Includes: 10mL Dynabeads oligo (dT)25, 60mL Lysis/Binding Buffer, 120mL Washing Buffer A, 60mL Washing Buffer B, 15mL 10mM Tris-HCl (Elution buffer), Contains enough reagents for 40 standard isolations.
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Descrizione

Descrizione

The Dynabeads™ mRNA DIRECT™ Kit is designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues. The isolated mRNA is suitable for use in all downstream applications.

Advantages of the Dynabeads mRNA DIRECT Kit:
• Fast—15 minute procedure yields pure, intact mRNA
• Highly pure mRNA isolation—best choice upstream of cDNA synthesis
• Sensitive mRNA isolation—enables cDNA synthesis and cDNA library construction from ultra-small starting samples (enables cDNA library construction from a single cell)

System overview
The isolation protocol relies on base pairing between the polyA residues at the 3' end of most mRNA, and the oligo (dT)25 residues covalently coupled to the surface of the Dynabeads. Other RNA species lacking a polyA tail will not hybridize to the beads and are readily washed away. Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, micro RNA, and small nucleolar RNA) do not bind to the beads and are discarded. RNase inhibiting agents in the Lysis/Binding Buffer together with stringent hybridization and washing conditions ensure the isolation of pure, intact mRNA from crude samples rich in RNase, without the use of strong chaotropic agents. The mRNA purification beads specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples (see protocol). 1 mg of Dynabeads Oligo (dT)25 beads (200 μL) binds up to 2 μg of mRNA. A typical mammalian cell contains about 10–30 pg of total RNA, from which 1–5% is mRNA.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY
Specifica

Specifica

• 10 mL Dynabeads Oligo (dt)25, 4°C
• 60 mL Lysis/Binding Buffer; 4°C
• 120 mL Washing Buffer A; 4°C
• 60 mL Washing Buffer B; 4°C
• 15 mL 10 mM Tris-HCl; 4°C
10 to 100 μL
mRNA
Automated Liquid Handling Systems
Beads may be reused for multiple extractions
Room Temperature
2 μg mRNA per 200 μL of beads (Binding capacity)
Magnetic Bead
Cells, Plants, Plasma, Serum, Tissue, Total RNA, Blood
High-throughput Compatible
RT-PCR, qPCR, cDNA library construction, microarray analysis
10 mL
Cells: ≤106
Plant: ≤400 mg
Tissue: ≤200 mg
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Suggerimenti di prodotto

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Software & Data Analysis

Automation Scripts

KingFisher Flex Protocol mRNA File size: 4.7 kB
KingFisher Duo protocol mRNA File size: 4.7 kB

Product Information

Manuals

Dynabeads mRNA DIRECT Kit User Guide Version: 009 File size: 434.4 kB
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Lot Number Certificate Type Date Catalog Number
Lot Number3043147 Certificate TypeCertificate of Analysis Date12/02/2025 Catalog Number61012
Lot Number3149114 Certificate TypeCertificate of Analysis Date12/02/2025 Catalog Number61012
Lot Number2793369 Certificate TypeCertificate of Analysis Date12/02/2025 Catalog Number61012
Lot Number2840184 Certificate TypeCertificate of Analysis Date12/02/2025 Catalog Number61012
Lot Number3041731 Certificate TypeCertificate of Analysis Date12/02/2025 Catalog Number61012
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Titolo del prodotto
Invitrogen™ Dynabeads™ mRNA DIRECT™ Purification Kit > 10 mL

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