Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads provide a fast, convenient method for purification of His-tagged recombinant proteins.
Brand: Pierce™ 78606
Product features:
Ni-NTA Magnetic Agarose Beads are used for small-scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). Tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates such as those prepared using Thermo Scientific B-PER Bacterial Protein Extraction reagents. Purification of His-tagged proteins is achieved using an NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites that allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Agarose Beads has been optimized to allow for high purity of the isolated His-tagged protein.
Applications:
| 5 mL | |
| Slurry: 25% | |
| Wet Ice | |
| Liquid Suspension | |
| 20 mL | |
| Magnetic Agarose Bead | |
| Bottle |
| 20 mL | |
| 5 mL settled resin. Supplied as 20 mL of 25% v/v suspension in 20% ethanol. Store at 2–8°C. |
|
| 30 μm | |
| Pierce™ | |
| Recombinant | |
| Metal Chelate, Divalent Nickel |
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For Research Use Only. Not for use in diagnostic procedures.
