General Western Blot Reagents
General Western Blot Reagents
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
Promotions are available
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Promotions are available
Thermo Scientific™ Pierce™ 1-Step Transfer Buffer
Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.
Invitrogen™ XCell II™ Blot Module
Easy transfer of proteins or nucleic acids from mini-gels to membranes
Invitrogen™ NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)
All buffers are made with high-purity reagents and are strictly quality controlled
Cytiva ECL Select Western Blotting Detection Reagent
Highly intense signal output for detection of medium to very low protein levels. Cytiva ECL Select Western Blotting Detection Reagent is a high sensitivity reagent for chemiluminescent Western blotting detection. Includes Luminol and Peroxide solutions, sufficient for 1000cm2 membrane.
Cytiva Amersham™ Reinforced Nitrocellulose Blotting Membrane
Nitrocellulose membrane, with a 0.45 µm pore size, is suitable for proteins of a wide range of molecular weights.
Thermo Scientific™ TMB Substrate Solution
A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)
Invitrogen™ Bolt™ Antioxidant
A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Invitrogen™ Tris-Glycine Transfer Buffer (25X)
Made with high-purity reagents and are strictly quality controlled
Invitrogen™ iBind™ Flex Solution Kit
Detection kit for use with iBind™ Flex Western System
Invitrogen™ No-Stain™ Protein Labeling Reagent
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
Invitrogen™ WesternBreeze™ Blocker/Diluent (Part A and B)
Optimized, easy-to-use blocker and primary antibody diluent system
Invitrogen™ Incubation Tray, 10 x 14 cm
Enables convenient and even staining of E-PAGE™ blots
Invitrogen™ NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)
Designed for separation of small- to medium-size proteins on NuPAGE Novex Bis-Tris gels