ELISA Substrates and Reagents

Colorimetric, chemiluminescent and chemifluorescent substrates for alkaline phosphatase (AP), horseradish peroxidase (HRP) and other enzyme reporter systems; includes several sensitive formulations of TMB for kinetic, competition or sandwich ELISA.

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Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

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Specifications

Substrate Properties	Chemical Substrate
Product Type	ELISA Substrate
Substrate Type	HRP (Horseradish Peroxidase)
Detection Method	Colorimetric

Invitrogen™ ELISA Stop Solution

A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.

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Specifications

Shipping Condition	Ambient
Content And Storage	Store at 2°C to 8°C
Form	Liquid
Product Type	ELISA Stop Solution
Substrate Type	HRP, TMB

Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

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Specifications

Substrate Properties	Chemical Substrate
Product Type	ELISA Substrate
Substrate Type	Alkaline Phosphatase
Detection Method	Colorimetric

Thermo Scientific™ Stop Solution for TMB Substrates

Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

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Thermo Scientific™ ELISA Carbonate Coating Buffer

For use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA

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Invitrogen™ ELISA Buffer Kit

Concentrated

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Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

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Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

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Thermo Scientific™ ELISA Blocker Blocking Buffer

Increases signal-to-noise ratio and minimizes background

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BD TMB Substrate Reagent Set

For use in BD OptEIA™ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is colorless to a very light amber solution containing 3,3', 5,5' tetramethylbenzidine (TMB) in an organic solvent.

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Invitrogen™ CSPD™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block™ Enhancer

Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.

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Invitrogen™ CSPD™ Substrate (0.25 mM Ready-To-Use)

This CSPD™ substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes.

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Specifications

Shipping Condition	Room Temperature
Content And Storage	Store the product at 4°C.
Format	Bottle,Bottle
Form	Liquid
Product Type	ELISA Substrate
Substrate Type	Alkaline Phosphatase
Detection Method	Chemiluminescence