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Thermo Scientific™ FastDigest MspI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Brand: Thermo Scientific™ FD0544

51.84 EUR valid until 2025-04-18
Use promo code "24107" to get your promotional price.

Product Code. 10630461

72.00 € / 400 reactions

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This item is not returnable. View return policy

Description

Lambda DNA digested with MspI, 1.4% agarose, 328 cleavage sites

FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment (Cat. No. EP0051), T4 DNA Ligase (Cat. No. EL0014), alkaline phosphatases and T4 DNA Polymerase (Cat. No. EP0061) all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.

Spécification

Content And Storage	• FastDigest MspI (400 μL) • 10X FastDigest Buffer (1.0 mL) • 10X FastDigest Green Buffer (1.0 mL) Store at –20°C.
Enzyme	MspI
Sensitive to Heat Inactivation	No
Type IIS RE	No
Product Type	Restriction Enzyme
Product Line	FastDigest™

Methylation Sensitivity	Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature	37°C
Quantity	400 μL (400 Reactions)
Research Category	Traditional Cloning
Overhang	5' protruding ends

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Documents

Product Information

Manuals

User Guide: Reaction Set-up for Digestion of Multiple DNA Samples Version: Jan. 2015 File size: 71.4 kB

User Guide: Scaling up DNA Digestion Reaction Version: Jan. 2015 File size: 60.9 kB

User Guide: FastDigest MspI Version: Jan. 2015 File size: 52 kB

User Guide: Double and Multiple Digestion of DNA Version: Jan. 2015 File size: 71.3 kB

User Guide: Fast Digestion of DNA Version: Jan. 2015 File size: 67.9 kB

Scientific Resources

Reference Materials

LabAid: FastDigest restriction enzymes File size: 62.2 kB

Certificats

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Lot Number

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Lot Number	Certificate Type	Date	Catalog Number
Lot Number2820419	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberFD0544
Lot Number2874420	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberFD0544
Lot Number2960567	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog NumberFD0544
Lot Number3015720	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog NumberFD0544
Lot Number3059955	Certificate TypeCertificate of Analysis	Date25/01/2025	Catalog NumberFD0544

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