IBA Lifesciences

Reversible capture and analysis of Twin-Strep-tag™ fusion proteins for Bio-Layer Interferometry (BLI) or Surface Plasmon Resonance (SPR) experiments

• Indirect immobilization of the ligand prevents it from inactivation or influences of the immobilization procedure
• High specificity and affinity in low pM range of the Twin-Strep-tag™ - Strep-Tactin™ XT interaction
• Ligands can be captured directly from complex samples
• Strep-Tactin™ XT biosensors/chips can be easily regenerated by an established procedure, whereby further ligand-analyte interactions can be analyzed with the same biosensor/chip

MagStrep 'Type 3' XT Magnetic Beads offer:

• High specificity and low background resulting in high protein purity
• High binding affinity and capacity for Strep-tag™ II or Twin-Strep-tag™ fusion proteins
• Immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) in batch format and/or high throughput screening for protein purification via pull-downs
• Suitable for usage with (semi-)automated magnetic bead purification systems (e.g., Thermo Scientific™ KingFisher™) for automated workflows

Strep-Tactin™ XT 4Flow™ Starter Kit consisting of everything you need for a quick start:

• Strep-Tactin™ XT 4Flow™ gravity flow column
• All buffers required for washing, elution and regeneration
• Strep-Tactin™ conjugated with HRP (for Western blot detection)
• Short protocol

Applicable for purification, detection and immobilization of biotinylated molecules

• Suitable for proximity-dependent Biotin Identification (BioID) experiments
• Mild and highly specific elution facilitating analysis of protein complexes

• Strep-Tactin™ Sepharose™ and Strep-Tactin™ Superflow™ for gravity flow column-based affinity purification

• MagStrep 'Type 3' Strep-Tactin™ magnetic beads are suitable for batch purification as well as pull-down assays

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection

• High purity, yield and viability
• Label-free, non-activated cells due to fully reversible binding
• From whole blood, buffy coat, PBMCs or murine samples
• Affinity chromatographic, magnetic or fluorescent cell separation

Detection and isolation of antigen-specific T-cells with MHC I Streptamers™ 

• Label-free cell isolation according to antigen specificity
• High specificity, sensitivity and reproducibility
• From single cell suspensions (e.g., PBMCs or murine samples)
• Affinity chromatographic, magnetic or fluorescent cell separation

Detection of antigen-specific T-cells with MHC I Streptamers™ and of receptor-specific T-cells with Fab Streptamers™

• Fully reversible
• High specificity, sensitivity and reproducibility
• No leftover staining reagents on the cells after FACS sorting
• Optimal preservation of cell effector function
• Improved viability of cells

More than 150 Twin-Strep-tag™/Strep-tag™ II expression vectors

• Variety of Twin-Strep-tag™/Strep-tag™ II combined with His, FLAG or GST
• Different N-/C-terminal orientations
• Expression hosts: E. coli, mammalia, insect cells, yeast
• Easy cloning via StarGate vectors

The MEXi mammalian expression system

Optimised for efficient protein expression and Strep-tag™ purification in mammalian cells.

• Perfectly matched HEK293E cell line and cell culture media
• Transient expression for time and cost efficient protein production
• Multiple expression vectors from IBA with different features

Strep-tag™ technology from the original manufacturer

• High affinity in low pM range
• Rapid one-step purification with unparalleled protein purity (>95%)
• Mild and specific elution
• Tolerates broad range of buffer conditions
• Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

For Western blot analysis, immunofluorescence, FACS and ELISA

Two different detection systems are provided:

• Strep-Tactin™ and Strep-Tactin™ XT conjugate for Twin-Strep-tag™/Strep-tag™ II proteins
• Monoclonal antibodies against Twin-Strep-tag™/Strep-tag™ II

Near covalent binding affinity – Immobilisation by Strep-Tactin XT: Twin-Strep-tag for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore) or BLI

• High affinity in low pM range enables measurements over a long time
• Minimal non-specific binding 
• Minimal coefficients of variation (cv)
• Chip for SPR experiments can be easily regenerated

Quick and simple isolation of unlabeled exosomes using Fab-TACS™ for exosomes, which offers:

• Highly specific and reproducible isolation
• Minimal contamination by other EVs
• Label-free exosomes with intact biological functions
• Source-independent application
• Reversible capture and release of exosomes
• A quick and simple protocol, without magnetic beads or ultracentrifugation