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Thermo Scientific™ Tango™ ADRA2A-bla U2OS DA Assay Kit

Brand: Thermo Scientific™ K1607

Product Code. 10647574

842.00 € / Each

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Description

The Tango™ ADRA2A-bla U2OS cells contain the human Adrenergic, Alpha-2A-, Receptor (ADRA2A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ ADRA2A-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ ADRA2A-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

Specifications

Accession Number	NM_000681.2
Cell State	Division-Arrested Cells
Cytoplasmic Tail	Tail from AVPR2 GPCR
Detection Method	Fluorescence
Gene Symbol	ADRA2A
Population Status	Single Cell Clone Origin
Quantity	8 x 10⁶ Cells
Sample Type	U2OS
Type	Division-Arrested Cells Assay Kit
System Type	Tango™

Assay Entry	Cell-based beta-arrestin recruitment
Content And Storage	Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: • Tango™ ADRA2A-bla U2OS DA cells • LiveBLAzer™-FRET B/G Loading Kit, 70 μg • Solution D • Tango™ ADRA2A-bla U2OS DA Assay Protocol • LiveBLAzer™-FRET B/G Loading Protocol • Certificate of Analysis The Tango™ ADRA2A-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Description	Tango™ ADRA2A-bla U2OS DA Assay Kit, 8 x 106 Cells, ∽4,800,000 cells/vial at 4,800,000 cells/mL Quantity of Cells, U2OS Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
For Use With (Application)	Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
Includes	Tango™ ADRA2A-bla U2OS DA assay kit Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: Tango™ ADRA2A-bla U2OS DA cells, LiveBLAzer™-FRET B/G loading kit, 70μg, solution D, Tango™ ADRA2A-bla U2OS DA assay protocol, LiveBLAzer™-FRET B/G loading protocol, certificate of analysis
Product Line	Tango™
Reporter Gene	BLA (Beta-Lactamase)
Target Entry	ADRA2A
Cell Line	U2OS
Druggable Target	GPCR

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Documents

Product Information

Manuals

User Guide: Tango ADRA2A‑bla U2OS DA and Dividing Cell‑based Assay Version: 2.0 File size: 783.3 kB

Tango™ ADRA2A-bla U2OS Cell Line Validation Packet Version: version 1.1 File size: 46.4 kB

Certificates

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Lot Number

2 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number1671203	Certificate TypeCertificate of Analysis	Date15/04/2017	Catalog NumberK1607
Lot Number1637857	Certificate TypeCertificate of Analysis	Date15/04/2017	Catalog NumberK1607

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