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Life Science Buffers

Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

  • Temperature
  • Desired or target pH
  • Buffer toxicity (to the system)
  • Buffer interactions with other system components

 

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1 – 15 of 55 results
1

TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents

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EDGE
Color Colorless
Physical Form Liquid
Chemical Name or Material Tris-EDTA
Grade Molecular Biology
DNase DNase free
Filtered Through Filtered through a 5-micron filter.
ChemAlert Storage Symbol Gray
Name Note 1X Solution, pH 8.0
CAS 7732-18-5
Protease Protease free
Health Hazard 3 Emergency Overview
May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention.

NFPA

Health:1
Flammability:0
Instability:0
Health Hazard 2 CAUTION!
pH 7.4 to 8.1
Synonym TE
Recommended Storage RT
2

Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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EDGE
PubChem CID 6503
CAS 77-86-1
Molecular Weight (g/mol) 121.136
ChEBI CHEBI:9754
SMILES C(C(CO)(CO)N)O
Synonym Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula C4H11NO3
3

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™

Commonly used buffering agent.

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EDGE
Molecular Weight (g/mol) 238.30
ChEBI CHEBI:42334
Color White
Chemical Name or Material HEPES
Grade Molecular Biology
SMILES OCCN1CCN(CCS(O)(=O)=O)CC1
Identification Pass Test
DNase DNase free
Merck Index 15, 4689
InChI Key JKMHFZQWWAIEOD-UHFFFAOYSA-N
ChemAlert Storage Symbol Gray
Assay Percent Range ≥99 %
PubChem CID 23831
Absorbance 0.01 max. (0.1M solution) at 280nm
Percent Purity ≥99%
CAS 7365-45-9
Protease Protease free
Health Hazard 3 Emergency Overview
Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. .

NFPA

Health:2
Flammability:1
Instability:1
MDL Number MFCD00006158
Health Hazard 2 WARNING!
Solubility Information Soluble in water
pH 5.0 to 6.5
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
Molecular Formula C8H18N2O4S
4

MOPS (Fine White Crystals/Molecular Biology), Fisher BioReagents™

CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N Synonym: 3-(4-Morpholino)propane sulfonic acid PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: 3-morpholin-4-ylpropane-1-sulfonic acid SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1

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EDGE
PubChem CID 70807
CAS 1132-61-2
Molecular Weight (g/mol) 209.26
ChEBI CHEBI:44115
MDL Number MFCD00006183
SMILES [O-]S(=O)(=O)CCC[NH+]1CCOCC1
Synonym 3-(4-Morpholino)propane sulfonic acid
IUPAC Name 3-morpholin-4-ylpropane-1-sulfonic acid
InChI Key DVLFYONBTKHTER-UHFFFAOYSA-N
Molecular Formula C7H15NO4S
5

Tris-HCl, 1M Solution, pH 8.0, Molecular Biology Grade, Ultrapure, Thermo Scientific Chemicals

CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl

EDGE
PubChem CID 93573
CAS 1185-53-1
Molecular Weight (g/mol) 157.594
SMILES C(C(CO)(CO)N)O.Cl
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
InChI Key QKNYBSVHEMOAJP-UHFFFAOYSA-N
Molecular Formula C4H12ClNO3
6

TBS, Tris Buffered Saline, 10X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™

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EDGE
Boiling Point 100°C
Color Colorless
Physical Form Liquid
Chemical Name or Material Tris Buffered Saline
Grade Molecular Biology
DNase DNase free
ChemAlert Storage Symbol Gray
Name Note 10X Solution
Concentration or Composition (by Analyte or Components) 10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl.
CAS 7732-18-5
Protease Protease free
pH 7.5
Synonym TBS
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
7

Corning™ Molecular Biology Reagents
BIOPHARMA

Used in molecular biology, nucleic acid purification applications

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8

PBS, 10X Solution, Molecular Biology Grade, Thermo Scientific Chemicals

For biological research. Thermo Scientific™ PBS, 10X Solution, Molecular Biology Grade is 0.2μm filtered and dispensed into durable, square nalgene bottles and upright carton.

EDGE
Form Solution
Formulation 80.6 mM sodium phosphate, 19.4 mM potassium phosphate, 27 mM KCl and 1.37 M NaCl in high purity dH2O
pH 7.4
9

HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™

Commonly used buffering agent

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EDGE
Color Undesignated
Physical Form Liquid
Chemical Name or Material HEPES Buffer
Grade Molecular Biology
Identification Pass Test
DNase DNase free
Merck Index 15, 4689
Concentration 0.95 to 1.05 M
ChemAlert Storage Symbol Gray
Absorbance ≤0.01(0.1M solution) at 260nm,0.004 max. (0.1M solution) at 280nm
Name Note 1M Solution, pH 7.3
CAS 7732-18-5
Protease Protease free
pH 7.2 to 7.5
Synonym N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
Molecular Formula C8H18N2O4S
Formula Weight 238.197
10

TE Buffer, 1X Solution pH 8.0, Low EDTA, Molecular Biology Grade, Thermo Scientific Chemicals

CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl

PubChem CID 93573
CAS 1185-53-1
Molecular Weight (g/mol) 157.594
SMILES C(C(CO)(CO)N)O.Cl
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
InChI Key QKNYBSVHEMOAJP-UHFFFAOYSA-N
Molecular Formula C4H12ClNO3
11

Thermo Scientific Chemicals Tris(hydroxymethyl)aminomethane, Molecular Biology Grade, 99.9%

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: TRIS PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

PubChem CID 6503
CAS 77-86-1
Molecular Weight (g/mol) 121.136
ChEBI CHEBI:9754
MDL Number MFCD00004679
SMILES C(C(CO)(CO)N)O
Synonym TRIS
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula C4H11NO3
12

Thermo Scientific Chemicals HEPES sodium salt, 99+%, for molecular biology, DNAse, RNAse and Protease free

CAS: 75277-39-3 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.28 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid sodium salt PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1

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PubChem CID 2724248
CAS 75277-39-3
Molecular Weight (g/mol) 260.28
ChEBI CHEBI:46758
MDL Number MFCD00036463
SMILES [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1
Synonym 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid sodium salt
IUPAC Name sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate
InChI Key RDZTWEVXRGYCFV-UHFFFAOYSA-M
Molecular Formula C8H17N2NaO4S
13

TAE Buffer, 10X Solution, Molecular Biology Grade, Ultrapure, Thermo Scientific Chemicals

Physical Form Solution
Chemical Name or Material TAE Buffer
Grade Molecular Biology, Ultrapure
TSCA Yes
Recommended Storage Ambient temperatures
Concentration 10X
14

Thermo Scientific Chemicals MOPS, 99.5%, for molecular biology, Dnase, Rnase, Prot. free, for peptide sequencing

CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N Synonym: 3-(N-Morpholino)propanesulfonic acid PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: 3-morpholin-4-ylpropane-1-sulfonic acid SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1

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PubChem CID 70807
CAS 1132-61-2
Molecular Weight (g/mol) 209.26
ChEBI CHEBI:44115
MDL Number MFCD00006183
SMILES [O-]S(=O)(=O)CCC[NH+]1CCOCC1
Synonym 3-(N-Morpholino)propanesulfonic acid
IUPAC Name 3-morpholin-4-ylpropane-1-sulfonic acid
InChI Key DVLFYONBTKHTER-UHFFFAOYSA-N
Molecular Formula C7H15NO4S
15

Thermo Scientific Chemicals HEPES free acid, 99+%, for molecular biology, DNase, Rnase and Protease free

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

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PubChem CID 23831
CAS 7365-45-9
Molecular Weight (g/mol) 238.30
ChEBI CHEBI:42334
MDL Number MFCD00006158
SMILES OCCN1CCN(CCS(O)(=O)=O)CC1
Synonym 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid
IUPAC Name 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
InChI Key JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula C8H18N2O4S